ns proteins การใช้

• The virus'11.5 nucleocapsid N protein, and the nonstructural NSs and NSm proteins.
• N protein encapsidates genome RNA into a nuclease-resistant form to protect in from cellular RNAse function.
• In general, for n proteins, the dynamical system takes the following form where x is typically protein concentration:
• Regarding the helical character of NSV RNPs, the main determinant of this conformation is the NP / N protein.
• For example, N protein is needed too much for the virus, because it coats outside of the replicated genomes completely.
• When discussing the NSV RNP, the NP / N protein is considered to be the most abundant element of the ribonucleoprotein.
• This is probably due to the advantage of having all RNA bound by N protein ( since N binds hexamers of RNA ).
• Genomic replication requires a source of newly synthesized N protein to encapsidate the RNA . This occurs during its synthesis and results in the production of a full length anti-genomic copy.
• The RSV genomic RNA forms a helical ribonucleoprotein ( RNP ) complex with the N protein, termed nucleocapsid, which is used as template for RNA synthesis by the viral polymerase complex.
• Deviation from the rule of six, through mutation or incomplete genome synthesis, leads to inefficient viral replication, probably due to structural constraints imposed by the binding between the RNA and the N protein.
• Assays are created that combine reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluorescence microscopy to silence the T6SS gene cluster by the histone-like nucleoid structuring H-NS protein.
• This reassortment can produce viral subtypes with combinations of genetic material unlike any our immune systems are used to, with genes that code for completely new H or N proteins, and maybe other proteins as well.
• In terms of the structure of the nucleocapsid, the N protein will eventually oligomerize to encapsidate the single-strand RNA . The N subunits are oriented in a parallel orientation, and the ssRNA is sequestered in the center.
• Because of having the N protein sequence at the beginning of the genome ( 3'end ) after the leader RNA sequence, mRNAs for N protein can always be produced and accumulate in high amounts with every termination of transcription.
• Because of having the N protein sequence at the beginning of the genome ( 3'end ) after the leader RNA sequence, mRNAs for N protein can always be produced and accumulate in high amounts with every termination of transcription.
• Within virions, the genomic RNAs of hantaviruses are thought to complex with the N protein to form helical nucleocapsids, the RNA component of which circularizes due to sequence complementarity between the 5'and 3'terminal sequences of genomic segments.